diff --git a/fastapi/applications.py b/fastapi/applications.py
index 568886f319..adbcb182c2 100644
--- a/fastapi/applications.py
+++ b/fastapi/applications.py
@@ -1187,7 +1187,7 @@ class FastAPI(Starlette):
         generate_unique_id_function: Callable[[routing.APIRoute], str] = Default(
             generate_unique_id
         ),
-        middleware: Optional[Sequence[Middleware]] = None,
+        middleware: Sequence[Middleware] | None = None,
     ) -> None:
         self.router.add_api_route(
             path,
@@ -1245,7 +1245,7 @@ class FastAPI(Starlette):
         generate_unique_id_function: Callable[[routing.APIRoute], str] = Default(
             generate_unique_id
         ),
-        middleware: Optional[Sequence[Middleware]] = None,
+        middleware: Sequence[Middleware] | None = None,
     ) -> Callable[[DecoratedCallable], DecoratedCallable]:
         def decorator(func: DecoratedCallable) -> DecoratedCallable:
             self.router.add_api_route(
@@ -1896,7 +1896,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -2285,7 +2285,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -2679,7 +2679,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -3073,7 +3073,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -3462,7 +3462,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -3851,7 +3851,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -4240,7 +4240,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -4634,7 +4634,7 @@ class FastAPI(Starlette):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
diff --git a/fastapi/routing.py b/fastapi/routing.py
index 5edac07de6..b5f517f1ff 100644
--- a/fastapi/routing.py
+++ b/fastapi/routing.py
@@ -1274,7 +1274,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(True),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this router.
@@ -1474,7 +1474,7 @@ class APIRouter(routing.Router):
         generate_unique_id_function: Callable[[APIRoute], str] = Default(
             generate_unique_id
         ),
-        middleware: Optional[Sequence[Middleware]] = None,
+        middleware: Sequence[Middleware] | None = None,
     ) -> Callable[[DecoratedCallable], DecoratedCallable]:
         def decorator(func: DecoratedCallable) -> DecoratedCallable:
             self.add_api_route(
@@ -2192,7 +2192,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -2585,7 +2585,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -2983,7 +2983,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -3381,7 +3381,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -3774,7 +3774,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -4167,7 +4167,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -4565,7 +4565,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.
@@ -4963,7 +4963,7 @@ class APIRouter(routing.Router):
             ),
         ] = Default(generate_unique_id),
         middleware: Annotated[
-            Optional[Sequence[Middleware]],
+            Sequence[Middleware] | None,
             Doc(
                 """
                 List of middleware to apply to all requests handled by this route.